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EBV DNA的检测方法及其在鼻咽癌诊治中的价值

2018年11月19日
编译:肿瘤资讯
来源:肿瘤资讯

EBV DNA的检测方法及其在鼻咽癌诊治中的价值

               
邵建永
教授

中山大学肿瘤防治中心
分子诊断科主任
防癌体检中心主任

随着IMRT广泛应用和放化疗联合的开展,鼻咽癌疗效有了较大提高,EBV-DNA检测则可以作用于鼻咽癌的早期筛查、疗效评价和复发监测,帮助临床医生作出更好地作出决策。邵建永教授的这次讲课将展示中山大学附属肿瘤医院EBV-DNA检测方法的发展情况,从2005年开始,EBV-DNA检测在我院飞速发展,从手工操作到自动提取血浆DNA,我们实现了更短时间,更多样品,更少错误地检测EBV-DNA,同时我们有严格的质控系统检测EBV-DNA的质量,极大地减轻了工作负担,提高了检测质量。我们的EBV-DNA检测方法效率优于其他商业试剂,并通过了斯坦福大学医学中心的EBV-DNA检测,我院实验室将成为中国的一项国际临床试验NRG-HN001的EBV-DNA检测中心实验室之一。

EBV-DNA Assay in SYSUCC and its Value in Management of NPC

With widespread use of IMRT and the combination of radiotherapy and chemotherapy, the curative effect of nasopharyngeal carcinoma has been greatly improved. EBV-DNA assay can act on the early screening, efficacy evaluation and recurrence monitoring of nasopharyngeal carcinoma, helping clinicians to make better decisions. Today, Professor Jian-Yong Shao will show the development of EBV-DNA assays of Sun Yat-sen University Cancer Center, starting from 2005. EBV DNA assays rapidly developed in our hospital. From manual to automatic, we implemented an assay with shorter time, more samples, less errors to detect EBV DNA. At the same time, we have strict quality control system to detect the quality of EBV-DNA, which greatly reduces the workload and improve the quality of the detection. Our EBV-DNA assay reagent diagnostic efficiency is prior to commercial reagents, and has passed the proficiency testing of EBV-DNA assay by the Stanford University Medical Center. Our labs will be one of EBV-DNA test central labs for the international NRG-HN001 clinical trial in China.